1. Applicable scenarios and blood collection sites

Applicable testing items: blood routine, blood type, blood glucose, erythrocyte sedimentation rate (Pan's method), microhematocrit, neonatal screening, etc.

Recommended blood sampling site:

Adults and children: left ring finger (due to less callus formation and moderate tissue thickness);

You can also choose the middle finger, avoiding the thumb, index finger (prone to calluses), and little finger (thin tissue);

Infants and young children: The heel is a commonly used part.

II. Preparation before operation

Personnel preparation: Operators should wear sterile gloves and perform hand hygiene before and after blood collection.

Item preparation:

Safety-type peripheral blood collector (touch-pressure or press-type, disposable);

Micro-peripheral blood sampling tube (usually EDTA-K₂ anticoagulant violet cap tube, used for blood routine examination, etc.);

75% ethanol or isopropanol cotton balls (prohibited for infants under 2 months old, to avoid contamination of the sample with iodophor);

Sterile dry cotton swabs, adhesive bandages, sharps containers, etc.

Environmental requirements: Adequate lighting, suitable temperature, clean and quiet.

III. Standard Operating Procedures

Identity verification: Two people should verify the patient's information to ensure it is consistent with the doctor's order.

Explanation and communication: Clarify the purpose, process, and potential discomforts to alleviate the patient's tension.

Disinfection:

Centered around the puncture point, perform spiral disinfection for ≥2 rounds, with a range of >5 cm;

Perform puncture after the disinfectant has fully evaporated.

Puncture:

The blood lancet is inserted at an angle of 30°–45° to the skin, with a depth of 2–3 mm;

After rapid puncture, gently and intermittently press the surrounding tissue to increase blood flow.

Blood collection:

Discard the first drop of blood (which may contain tissue fluid diluting the sample);

The blood collection tube will be brought into contact with the second drop of blood, maintaining an angle of 30°–45°, allowing the blood to flow along the tube wall;

If a micro pipette is used, it automatically aspirates through siphon action, avoiding air bubbles.

Hemostasis: After the procedure, remove the needle and immediately apply pressure with a dry cotton swab to stop bleeding.

Mixing and labeling:

Immediately invert and gently mix the blood collection tube 8 times (EDTA anticoagulant tube)

The label is clear, unique, and traceable